Thank you!

Your quote has been successfully submitted!

For products requiring additional information, our team will contact you within 1 business day

Failed

There was an error submitting your quote. Please try again.

Recombinant Human Contactin 3/CNTN3 Protein (708 Asp/Asn, His Tag)(Act– MSE Supplies LLC

Free Shipping on MSE PRO Online Orders of $500 or More! U.S. Orders Only * Offer Excludes Hazmat Shipments *

Menu

This product has been added to the cart.

Recombinant Human Contactin 3/CNTN3 Protein (708 Asp/Asn, His Tag)(Active)

SKU: PKSH031802-100

  • $ 65895
  • Save $ 7400



Recombinant Human Contactin 3/CNTN3 Protein (708 Asp/Asn, His Tag)(Active)

 

SKU # PKSH031802
Expression Host HEK293 Cells

 

 

Description

Synonyms BIG-1, PANG, PCS
Species Human
Expression Host HEK293 Cells
Sequence Met 1-Ser1002; 708 Asp/Asn
Accession NP_065923.1
Calculated Molecular Weight 109.2 kDa
Observed Molecular Weight 120-130 kDa
Tag C-His
Bio-activity Measured by the ability of the immobilized protein to support the adhesion of C6 Rat brain glial cells. When 5 x 104 cells/well are added to CNTN3 coated plates (0.8 μg/ml and 100 μl/well), approximately 35%-55% will adhere specifically after 60 minutes at 37℃.
  

 

Properties

Purity > 97 % as determined by reducing SDS-PAGE.
Endotoxin < 1.0 EU per μg of the protein as determined by the LAL method.
Storage Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping This product is provided as lyophilized powder which is shipped with ice packs.
Formulation Lyophilized from sterile PBS, pH 7.4
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution Please refer to the printed manual for detailed information.



Background

Contactins are a subgroup of molecules belonging to the immunoglobulin superfamily that are expressed exclusively in the nervous system. The subgroup consists of six members: Contactin-1; Contactin-2(TAG-1); Contactin-3(BIG-1); BIG-2; Contactin-5(NB-2) and NB-3. Since their identification in the late 1980s; Contactin-1 and Contactin-2 have been studied extensively. Axonal expression and the neurite extension activity of Contactin-1 and Contactin-2 attracted researchers to study the function of these molecules in axon guidance during development. Contactin-1 and Contactin-2 have come to be known as the principal molecules in the function and maintenance of myelinated neurons. In contrast; the function of the other four members of this subgroup remained unknown until recently. Contactin-3; also known as CNTN3 ( BIG-1 in rat and PANG in mouse ); is a GPI-linked glycoprotein that is expressed on cerebellar Purkinje cells; amygdaloid and thalamic neurons and olfactory granule cells. In the brain; Contactin-3 is expressed in frontal lobe; occipital lobe; cerebellum and amygdala. Contactin-3 contains 4 fibronectin type-III domains and 6 Ig-like C2-type (immunoglobulin-like) domains. Human Contactin-3 shares 92% aa identity with mouse Contactin-3.The exact function of Contactin-3 is unclear. Contactin-3 may mediate cell-cell interaction and may promote neurite outgrowth.