Recombinant Human Contactin 3/CNTN3 Protein (708 Asp/Asn, His Tag)(Active)
SKU: PKSH031802-100
Recombinant Human Contactin 3/CNTN3 Protein (708 Asp/Asn, His Tag)(Active)
SKU # | PKSH031802 |
Expression Host | HEK293 Cells |
Description
Synonyms | BIG-1, PANG, PCS |
Species | Human |
Expression Host | HEK293 Cells |
Sequence | Met 1-Ser1002; 708 Asp/Asn |
Accession | NP_065923.1 |
Calculated Molecular Weight | 109.2 kDa |
Observed Molecular Weight | 120-130 kDa |
Tag | C-His |
Bio-activity | Measured by the ability of the immobilized protein to support the adhesion of C6 Rat brain glial cells. When 5 x 104 cells/well are added to CNTN3 coated plates (0.8 μg/ml and 100 μl/well), approximately 35%-55% will adhere specifically after 60 minutes at 37℃. |
Properties
Purity | > 97 % as determined by reducing SDS-PAGE. |
Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
Formulation | Lyophilized from sterile PBS, pH 7.4 Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
Reconstitution | Please refer to the printed manual for detailed information. |
Background
Contactins are a subgroup of molecules belonging to the immunoglobulin superfamily that are expressed exclusively in the nervous system. The subgroup consists of six members: Contactin-1; Contactin-2(TAG-1); Contactin-3(BIG-1); BIG-2; Contactin-5(NB-2) and NB-3. Since their identification in the late 1980s; Contactin-1 and Contactin-2 have been studied extensively. Axonal expression and the neurite extension activity of Contactin-1 and Contactin-2 attracted researchers to study the function of these molecules in axon guidance during development. Contactin-1 and Contactin-2 have come to be known as the principal molecules in the function and maintenance of myelinated neurons. In contrast; the function of the other four members of this subgroup remained unknown until recently. Contactin-3; also known as CNTN3 ( BIG-1 in rat and PANG in mouse ); is a GPI-linked glycoprotein that is expressed on cerebellar Purkinje cells; amygdaloid and thalamic neurons and olfactory granule cells. In the brain; Contactin-3 is expressed in frontal lobe; occipital lobe; cerebellum and amygdala. Contactin-3 contains 4 fibronectin type-III domains and 6 Ig-like C2-type (immunoglobulin-like) domains. Human Contactin-3 shares 92% aa identity with mouse Contactin-3.The exact function of Contactin-3 is unclear. Contactin-3 may mediate cell-cell interaction and may promote neurite outgrowth.