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Recombinant Human Cystatin 7/CST7 Protein (His Tag)(Active)
SKU: PKSH031812-20
Recombinant Human Cystatin 7/CST7 Protein (His Tag)(Active)
| SKU # | PKSH031812 |
| Expression Host | HEK293 Cells |
Description
| Synonyms | CMAP, CST7, Cystatin-7, Cystatin-F, Cystatin-Like Metastasis-Associated Protein, Leukocystatin |
| Species | Human |
| Expression Host | HEK293 Cells |
| Sequence | Met 1-His 145 |
| Accession | NP_003641.3 |
| Calculated Molecular Weight | 16.1 kDa |
| Observed Molecular Weight | 44 kDa |
| Tag | C-His |
| Bio-activity | Measured by its ability to inhibit active Cathepsin L cleavage of a fluorogenic peptide substrate Z-LR-AMC (R&D Systems, Catalog # ES008). The IC50 value is < 6 nM. |
Properties
| Purity | > 92 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from sterile PBS, pH 7.4 Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
The cystatin superfamily members are important natural cysteine protease inhibitors present in a wide variety of organisms and are divided into three classes. Cystatin F, also known as leukocystatin and CMAP (Cystatin-like Metastasis-Associated Protein), is a type 2 cystatin and its expression is limited to hematopoietic cells, with the highest expression levels being observed in monocytes, dendritic cells, and certain types of T-cells. Furthermore, cystatin F mRNA becomes up-regulated during dendritic cell maturation, and thus suggests a specific role of cystatin F in immune regulation. Cystatin F is produced as a dimer, an inactive cathepsin inhibitor which is activated by chemical reduction. In addition, Cystatin F and its homologues have been observed expressing in various human cancer cell lines established from malignant tumors, and thus indicates a new diagnosis and prevention approach of certain human carcinomas metastasis.