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Recombinant Human Deoxyribonuclease 1/DNASE1 Protein (His tag)
SKU: PDMH100102-100
Recombinant Human Deoxyribonuclease 1/DNASE1 Protein (His tag)
| SKU # | PDMH100102 |
| Expression Host | HEK293 Cells |
Description
| Synonyms | DNASE1, DNL1, DNase I, DRNI, Deoxyribonuclease I, Deoxyribonuclease-1, Dornase alfa, EC 3.1.21.1 |
| Species | Human |
| Expression Host | HEK293 Cells |
| Sequence | Met 1-Lys282 |
| Accession | P24855 |
| Calculated Molecular Weight | 30.9 kDa |
| Observed Molecular Weight | 40 kDa |
| Tag | C-His |
| Bio-activity | Not validated for activity |
Properties
| Purity | > 95% as determined by reducing SDS-PAGE. |
| Endotoxin | Please contact us for more information. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from sterile PBS, pH 7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis. |
Background
DNase1, also known as deoxyribonuclease I and DNL1, is a member of the DNase family. DNaseI is a nuclease that cleaves DNA preferentially at phosphodiester linkages adjacent to a pyrimidine nucleotide, yielding 5'-phosphate-terminated polynucleotides with a free hydroxyl group on position 3', on average producing tetranucleotides. DNaseI binds to the cytoskeletal protein actin. It binds actin monomers with very high (sub-nanomolar) affinity and actin polymers with lower affinity. Mutations in DNase1 gene have been associated with systemic lupus erythematosus (SLE), an autoimmune disease. DNase1 is used to treat the one of the symptoms of cystic fibrosis by hydrolyzing the extracellular DNA in sputum and reducing its viscosity.