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Recombinant Human EIF1AX Protein (His Tag)
SKU: PKSH032372-50
Recombinant Human EIF1AX Protein (His Tag)
| SKU # | PKSH032372 |
| Expression Host | E.coli |
Description
| Synonyms | EIF1A, EIF1AX, EIF4C, Eukaryotic Translation Initiation Factor 1A X-Chromosomal, Eukaryotic Translation Initiation Factor 4C, eIF-1A X Isoform, eIF-4C |
| Species | Human |
| Expression Host | E.coli |
| Sequence | Met 1-Ile144 |
| Accession | P47813 |
| Calculated Molecular Weight | 18.6 kDa |
| Observed Molecular Weight | 22 kDa |
| Tag | N-His |
| Bio-activity | Not validated for activity |
Properties
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
Eukaryotic Translation Initiation Factor 1A, X-Chromosomal (EIF1AX) is an essential eukaryotic translation initiation factor that belongs to the eIF-1A family. EIF1AX is required for the binding of the 43S complex (a 40S subunit, eIF2/GTP/Met-tRNAi and eIF3) to the 5' end of capped RNA and has been shown to interact with IPO13. EIF1AX contains one S1-like domain and seems to be required for maximal rate of protein biosynthesis. Enhances ribosome dissociation into subunits and stabilizes the binding of the initiator Met-tRNA(I) to 40 S ribosomal subunits.