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Recombinant Human eIF4E protein (GST,His tag)
SKU: PDEH100408-100
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Recombinant Human eIF4E protein (GST,His tag)
| SKU # | PDEH100408 |
| Expression Host | E. coli |
Description
| Synonyms | Eukaryotic translation initiation factor 4E;eIF-4E;eIF-4F 25 kDa subunit;mRNA cap-binding protein;EIF4E;EIF4EL1;EIF4F |
| Species | Human |
| Expression_host | E.coli |
| Sequence | Ala 2-Val 217 |
| Accession | P06730 |
| Mol_Mass | 48.7 kDa |
| AP_Mol_Mass | 52 kDa |
| Tag | N-GST & C-His |
| Bio_Activity | Not validated for activity |
Propertie
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin level | Please contact us for more information. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis |
Background
Eukaryotic translation initiation factor 4E is a 217 amino acids protein that belongs to the eukaryotic initiation factor 4E family. eIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least EIF4A, EIF4E and EIF4G1/EIF4G3. EIF4E is also known to interact with other partners.It can recognize and bind the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures.