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Recombinant Human EpCAM/TROP-1 Protein (His Tag)
SKU: PKSH032382-50
Recombinant Human EpCAM/TROP-1 Protein (His Tag)
| SKU # | PKSH032382 |
| Expression Host | HEK293 Cells |
Description
| Synonyms | Adenocarcinoma-Associated Antigen, Cell Surface Glycoprotein Trop-1, EGP, EGP314, Ep-CAM, Epithelial Cell Adhesion Molecule, Epithelial Cell Surface Antigen, Epithelial Glycoprotein, Epithelial Glycoprotein 314, KSA, Tumor-Associated Calcium Signal Transduc, hEGP314 |
| Species | Human |
| Expression Host | HEK293 Cells |
| Sequence | Gln24-Lys265 |
| Accession | AAH14785.1 |
| Calculated Molecular Weight | 28.4 kDa |
| Observed Molecular Weight | 35-45 kDa |
| Tag | C-His |
| Bio-activity | Not validated for activity |
Properties
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.2. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
Epithelial Cell Adhesion Molecule (EpCAM) is a signal type I transmembrane glycoprotein that belongs to the EPCAM family. EpCAM is composed of an extracellular domain with one thyroglobulin type-1 domain; a transmembrane domain and a cytoplasmic domain. EpCAM is found on the surface of adenocarcinoma; but not on mesodermal or neural cell membranes. The EpCAM molecule has been shown to function as a homophilic Ca2+ independent adhesion molecule. It may act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium as an immunological barrier providing the first line of defense against infection. Defects in EPCAM are a cause of hereditary non-polyposis colorectal cancer type 8 (HNPCC8) and diarrhea type 5 (DIAR5). EpCAM plays a role in embryonic stem cells proliferation and differentiation; it up-regulates the expression of FABP5; MYC and Cyclin A and Cyclin E. It is highly and selectively expressed by undifferentiated embryonic stem cells.