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Recombinant Human EphA4 Protein (aa 570-986, His &GST Tag)(Active)
SKU: PKSH030369-50
Recombinant Human EphA4 Protein (aa 570-986, His &GST Tag)(Active)
| SKU # | PKSH030369 |
| Expression Host | Baculovirus-Insect Cells |
Description
| Synonyms | EK8, EPH-like kinase 8, EPHA4, Ephrin type-A receptor 4, HEK8, SEK, TYRO1, Tyrosine-protein kinase TYRO1, Tyrosine-protein kinase receptor SEK, hEK8 |
| Species | Human |
| Expression Host | Baculovirus-Insect Cells |
| Sequence | Ser 570-Val 986 |
| Accession | P54764 |
| Calculated Molecular Weight | 75.0 kDa |
| Observed Molecular Weight | 67 kDa |
| Tag | N-His-GST |
| Bio-activity | 1. The specific activity was determined to be 17 nmol/min/mg using Poly(Glu:Tyr) 4:1 as substrate. 2. Immobilized human EPHA4 (aa 570-986)at 10 μg/ml (100 μl/well) can bind biotinylated human EphrinA5-His with a linear range of 0.625-5.0 μg/ml. |
Properties
| Purity | > 99 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles. |
| Shipping | This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C. |
| Formulation | Supplied as sterile solution of 20mM Tris, 500mM NaCl, pH 8.5, 10% glycerol, 3mM DTT |
| Reconstitution | Not Applicable |
Background
EPH receptor A4 (ephrin type-A receptor 4); also known as EphA4; belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family which 16 known receptors (14 found in mammals) are involved: EPHA1; EPHA2; EPHA3; EPHA4; EPHA5; EPHA6; EPHA7; EPHA8; EPHA9; EPHA10; EPHB1; EPHB2; EPHB3; EPHB4; EPHB5; EPHB6. The Eph family of receptor tyrosine kinases (comprising EphA and EphB receptors) has been implicated in synapse formation and the regulation of synaptic function and plasticity6. EphA4 is enriched on dendritic spines of pyramidal neurons in the adult mouse hippocampus; and ephrin-A3 is localized on astrocytic processes that envelop spines. Eph receptor−mediated signaling; which is triggered by ephrins7; probably modifies the properties of synapses during synaptic activation and remodeling. Ephrin receptors are components of cell signalling pathways involved in animal growth and development; forming the largest sub-family of receptor tyrosine kinases (RTKs). The extracellular domain of an EphA4 interacts with ephrin ligands; which may be tethered to neighbouring cells. Ligand-mediated activation of Ephs induce various important downstream effects and Eph receptors have been studied for their potential roles in the development of cancer.