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Recombinant Human EphB4/HTK Protein (Active)
SKU: PKSH031737-100
Recombinant Human EphB4/HTK Protein (Active)
| SKU # | PKSH031737 |
| Expression Host | HEK293 Cells |
Description
| Synonyms | HTK, MYK1, TYRO11 |
| Species | Human |
| Expression Host | HEK293 Cells |
| Sequence | Met 1-Ala 539 |
| Accession | NP_004435.3 |
| Calculated Molecular Weight | 57.7 kDa |
| Observed Molecular Weight | 65-70 kDa |
| Tag | None |
| Bio-activity | Immobilized human EphB4 at 2 μg/ml (100 μl/well) can bind human EphrinB2 with a linear range of 1-125 ng/ml. |
Properties
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from sterile 100mM NaCl, 50mM Tris, pH 7.5 Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
Ephrin type-B receptor 4 is a protein that in humans is encoded by the EPHB4 gene. It is a single-pass type I membrane protein belonging to the ephrin receptor subfamily of protein kinase superfamily. Members of the ephrin and Eph family are local mediators of cell function through largely contact-dependent processes in development and in maturity. Furthermore; EphB4 protein and the corresponding ligand Ephrin-B2 contribute to tumor growth in various human tumors. EphB4 protein has tumor suppressor activities and that regulation of cell proliferation; extracellular matrix remodeling; and invasive potential are important mechanisms of tumor suppression. Therefore; Ephrin-B2/EphB4 may be recognized as a novel prognostic indicator for cancers.