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Recombinant Human FLT-3/FLK-2 Protein (Fc Tag)
SKU: PKSH033543-50
Recombinant Human FLT-3/FLK-2 Protein (Fc Tag)
| SKU # | PKSH033543 |
| Expression Host | HEK293 Cells |
Description
| Synonyms | CD135, FL Cytokine Receptor, FLK-2, FLK2, FLT-3, FLT3, Fetal Liver Kinase-2, Fms-Like Tyrosine Kinase 3, Receptor-Type Tyrosine-Protein Kinase FLT3, STK-1, STK1, Stem Cell Tyrosine Kinase 1 |
| Species | Human |
| Expression Host | HEK293 Cells |
| Sequence | Asn27-Asn541 |
| Accession | AAI26351.1 |
| Calculated Molecular Weight | 85.3 kDa |
| Observed Molecular Weight | 120 kDa |
| Tag | C-Fc |
| Bio-activity | Not validated for activity |
Properties
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles. |
| Shipping | This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C. |
| Formulation | Supplied as a 0.2 μm filtered solution of PBS, pH7.4. |
| Reconstitution | Not Applicable |
Background
The Flt-3 (fms-like tyrosine kinase) receptor, also named Flk-2and Stk-1is a member of the class III subfamily of receptor tyrosine kinases that also includes KIT, the receptor for SCF and FMS, the receptor for M-CSF. The extracellular region of these receptors contains five immunoglobulin-like domains and the intracellular region contains a split kinase domain. Human Flt-3 cDNA encodes a 993 amino acid (aa) residue type I membrane protein with a 26 aa residue signal peptide, a 515 aa extracellular domain with 10 potential N-linked glycosylation sites, a 21 aa residue transmembrane domain and a 431 aa residue cytoplasmic domain. Flt-3 expression has been detected in various tissues, including placenta, gonads, and tissues of nervous and hematopoietic origin. Among hematopoietic cells, the expression of Flt-3 was found to be restricted to the highly enriched stem/progenitor cell populations. The ligand for Flt-3 (FL) has been identified to be a transmembrane protein with structural homology to M-CSF and SCF. Recombinant soluble Flt-3/Fc chimeric protein has been shown to bind FL with high affinity and is a potent FL antagonist.