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Recombinant Human GADD45A protein (His tag)
SKU: PDEH100303-100
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Recombinant Human GADD45A protein (His tag)
| SKU # | PDEH100303 |
| Expression Host | E. coli |
Description
| Synonyms | Growth arrest and DNA damage-inducible protein GADD45 alpha;GADD45A;DNA damage-inducible transcript 1 protein; |
| Species | Human |
| Expression_host | E.coli |
| Sequence | Met 1-Arg 165 |
| Accession | P24522 |
| Mol_Mass | 18.0 kDa |
| AP_Mol_Mass | 23 kDa |
| Tag | N-His |
| Bio_Activity | Not validated for activity |
Properties
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin level | Please contact us for more information. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis |
Background
GADD45 alpha (Growth arrest and DNA damage-inducible protein 45 alpha) is a 19 kDa member of the GADD45 family of proteins. It is ubiquitously expressed, and induced by DNA damage. Upon induction, GADD45 alpha binds to PCNA, stimulates DNA excision repair, and initiates G2 cell cycle arrest. Human GADD45 alpha is 165 amino acids (aa) in length. It is reported to dimerize with GADD45-beta and -gamma, and to homo-dimerize, -trimerize and -tetramerize via residues 33?61 and 133?165. There is one splice variant that shows a Ser substitution for aa 14?49. GADD45 alpha is 55% aa identical to GADD45-beta and -gamma, and shares 94% aa identity with mouse GADD45 alpha.