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Recombinant Human Galectin-3/LGALS3 Protein (Active)
SKU: PKSH032474-50
Recombinant Human Galectin-3/LGALS3 Protein (Active)
| SKU # | PKSH032474 |
| Expression Host | E.coli |
Description
| Synonyms | 35 kDa Lectin, CBP35, Carbohydrate-Binding Protein 35, GAL3, GALBP, GALIG, Gal-3, Galactose-Specific Lectin 3, Galactoside-Binding Protein, Galectin-3, IgE-Binding Protein, L-31, L31, LGALS2, LGALS3, Laminin-Binding Protein, Lectin L-29, MAC2, Mac-2 Antigen, P35 |
| Species | Human |
| Expression Host | E.coli |
| Sequence | Ala2-Ile250 |
| Accession | P17931 |
| Calculated Molecular Weight | 26.0 kDa |
| Observed Molecular Weight | 30 kDa |
| Tag | None |
| Bio-activity | Not validated for activity |
Properties
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from a 0.2 μm filtered solution of 50mM HEPES, 5% Sucrose, 5% Mannitol, 0.06%Tween 80, pH7.5. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
The Galectin family of proteins consists of beta-galactoside binding lectins containing homologous carbohydrate recognition domains (CRDs). They also possess hemagglutination activity; which is attributable to their bivalent carbohydrate binding properties. Galectins are active both intracellularly and extracellularly. They have diverse effects on many cellular functions including adhesion; migration; polarity; chemotaxis; proliferation; apoptosis; and differentiation. Galectins may therefore play a key role in many pathological states; including autoimmune diseases; allergic reactions; inflammation; tumor cell metastasis; atherosclerosis; and diabetic complications. The galectins have been classified into the prototype galectins (1; 2; 5; 7; 10; 11; 13; 14); which contain one CRD and exist either as a monomer or a noncovalent homodimer. The chimera galectins (Galectin3) containing one CRD linked to a nonlectin domain; and the tandem repeat Galectins (4; 6; 8; 9; 12) consisting of two CRDs joined by a linker peptide. Galectins lack a classical signal peptide and can be localized to the cytosolic compartments where they have intracellular functions. However; via one or more as yet unidentified nonclassical secretory pathways; galectins can also be secreted to function extracellularly. Individual members of the galectin family have different tissue distribution profiles and exhibit subtle differences in their carbohydrate-binding specificities. Each family member may preferentially bind to a unique subset of cell surface glycoproteins.