Recombinant Human Grancalcin/GCA Protein (GST Tag)
SKU: PKSH032507-50
Recombinant Human Grancalcin/GCA Protein (GST Tag)
SKU # | PKSH032507 |
Expression Host | E.coli |
Description
Synonyms | GCA, GCL, Grancalcin |
Species | Human |
Expression Host | E.coli |
Sequence | Met 1-Ile217 |
Accession | P28676 |
Calculated Molecular Weight | 50.3 kDa |
Observed Molecular Weight | 50 kDa |
Tag | N-GST |
Bio-activity | Not validated for activity |
Properties
Purity | > 95 % as determined by reducing SDS-PAGE. |
Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
Formulation | Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl,4% Sucrose,4% Mannitol,0.02% Tween 80 (w/v),pH 8.0. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
Reconstitution | Please refer to the printed manual for detailed information. |
Background
Grancalcin (GCA) is a member of the penta EF hand subfamily which includes sorcin; calpain and ALG2. Grancalcin is highly expressed bone marrow and also can detected in neutrophils and macrophages. Grancalcin interacts with L-plastin which known to have actin bundling activity. It indicates that Grancalcin may play an important role in the adhesion of neutrophils to fibronectin. Furthermore; Grancalcin localization is dependent upon calcium and magnesium. It associates with both the granule and membrane fractions; which suggested a role for grancalcin in granule-membrane fusion and degranulation.