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Recombinant Human GSS Protein (His Tag)
SKU: PKSH032497-50
Recombinant Human GSS Protein (His Tag)
| SKU # | PKSH032497 |
| Expression Host | E.coli |
Description
| Synonyms | GSH Synthetase, GSH-S, GSS, Glutathione Synthase, Glutathione Synthetase |
| Species | Human |
| Expression Host | E.coli |
| Sequence | Ala2-Val474 |
| Accession | P48637 |
| Calculated Molecular Weight | 53.5 kDa |
| Observed Molecular Weight | 55 kDa |
| Tag | C-His |
| Bio-activity | Not validated for activity |
Properties
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles. |
| Shipping | This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C. |
| Formulation | Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 200mM NaCl, pH 7.5. |
| Reconstitution | Not Applicable |
Background
Glutathione Synthetase belongs to the eukaryotic GSH synthase family. Glutathione Synthetase is the second enzyme in the glutathione biosynthesis pathway. It catalyses the condensation of gamma-glutamylcysteine and glycine to form glutathione. Glutathione play an important role in a variety of biological functions, including detoxification of xenobiotics, protection of cells from oxidative damage by free radicals, and membrane transport. The protein functions as a homodimer to catalyze the second step of glutathione biosynthesis, which is the ATP-dependent conversion of gamma-L-glutamyl-L-cysteine to glutathione. Defects in Glutathione Synthetase can also cause the glutathione synthetase deficiency of erythrocytes, which is a mild form causing hemolytic anemia.