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Recombinant Human Interleukin-18/IL-18 Protein (GST Tag)
SKU: PKSH030306-50
Recombinant Human Interleukin-18/IL-18 Protein (GST Tag)
| SKU # | PKSH030306 |
| Expression Host | E.coli |
Description
| Synonyms | MGC, IL1F, IL-1F, IL18, IGIF, IL-18, IL-1g, IL1F4, GIF, Iboctadekin, IFN-gamma-inducing factor, IL-1 gamma, IL-1F4, Interferon gamma-inducing factor, Interferon-gamma-inducing factor, Interleukin 18, Interleukin-1 gamma, Interleukin-18, MGC12320, IFN gamma inducing factor, IL 1 gamma, IL 18, IL 1g, IL1 gamma, IL18 protein, IL1g, IL1gamma, ILIF4, Interferon gamma inducing factor, Interleukin 1 gamma, Interleukin 18 (interferon-gamma-inducing factor), Interleukin18 |
| Species | Human |
| Expression Host | E.coli |
| Sequence | Met 1-Asp 193 |
| Accession | Q14116 |
| Calculated Molecular Weight | 48.6 kDa |
| Tag | N-GST |
| Bio-activity | Not validated for activity |
Properties
| Purity | > 85 % as determined by reducing SDS-PAGE. |
| Endotoxin | Please contact us for more information. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from sterile PBS, pH 7.5 Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
Interleukin-18 (IL-18; also known as interferon-gamma inducing factor) is a proinflammatory cytokine that belongs to the IL-1 superfamily and is produced by macrophages and other cells. This cytokine can induce the IFN-gamma production of T cells. The combination of IL-18 and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production; and enhance IgG2a production of B cells. IL-18 binding protein (IL18BP) can specifically interact with this cytokine; and thus negatively regulate its biological activity. IL-18 is an IL-1−like cytokine that requires cleavage with caspase-1 to become active; was found to increase IgE production in a CD4+ T cells-; IL-4− and STAT6−dependent fashion. IL-18 and T cell receptor−mediated stimulation could induce naïve CD4+ T cells to develop into IL-4−producing cells in vitro. Thus; caspase-1 and IL-18 may be critical in regulation of IgE production in vivo; providing a potential therapeutic target for allergic disorders. IL-18 production in primary synovial cultures and purified synovial fibroblasts was; in turn; upregulated by TNF-α and IL-1β; suggesting that monokine expression can feed back to promote Th1 cell development in synovial membrane. Besides; synergistic combinations of IL-18; IL-12; and IL-15 may be of importance in sustaining both Th1 responses and monokine production in RA.