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Recombinant Human Interleukin-36 alpha/IL-36 alpha
SKU: PKSH033873-100
Recombinant Human Interleukin-36 alpha/IL-36 alpha
| SKU # | PKSH033873 |
| Expression Host | E.coli |
Description
| Synonyms | FIL, IL1F, Interleukin-1 Family Member, IL-1F, IL36A, FIL1, FIL1(EPSILON), FIL1E, IL-1F6, IL1(EPSILON), IL1F6, FIL1 Epsilon, IL-1 Epsilon, Interleukin-1 Epsilon, Interleukin-1 Family Member 6, Interleukin-36 Alpha, IL1E, IL 1 epsilon, IL 1F6, IL 1H1, IL1RP2, Interleukin 1 epsilon, Interleukin 1 family member 6, Interleukin 1 family member 6 (epsilon), Interleukin 36 alpha, MGC129552, MGC129553, MGC151479, MGC151481, OTTMUSP00000012798, RP23-176J12.4 |
| Species | Human |
| Expression Host | E.coli |
| Sequence | Lys6-Phe158 |
| Accession | Q9UHA7 |
| Calculated Molecular Weight | 18.1 kDa |
| Observed Molecular Weight | 17 kDa |
| Tag | C-His |
| Bio-activity | Measure by its ability to induce IL-8 secretion in human PBMCs.The ED50 for this effect is <0.7 ng/mL. |
Properties
| Purity | > 98 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 0.1 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from sterile PBS, pH 7.4. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
Human Interleukin-36α (IL-36α) is a secreted cytokine that belongs to the Interleukin 1 cytokine family. IL-36α is expressed in the immune system and the fetal brain, but not in other tissues or multiple hematopoietic cell lines. IL-36α is the only IL-1 family member found to be expressed on T-cells. IL-36α and IL-1F8 are involved in the regulation of adipose tissue gene expression. Importantly, IL-36α inhibits PPARγ expression, which may lead to a reduction in adipocyte differentiation suggesting metabolic effects of this cytokine. IL-36α, along with IL-1F8 and IL-1F9, has been shown to act as an agonist by activating the pathway involving NFκB and MAPK in an IL-1Rrp2 dependent manner. This suggest that IL-36α may signal in a similar fashion to IL-1 and IL-18 in having a binding receptor which upon ligation, recruits a second receptor as a signaling component, forming an active heterodimeric receptor complex.