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Recombinant Human KLRB1/CD161 Protein (Fc Tag)
SKU: PKSH032676-50
Recombinant Human KLRB1/CD161 Protein (Fc Tag)
| SKU # | PKSH032676 |
| Expression Host | HEK293 Cells |
Description
| Synonyms | C-type lectin domain family 5 member B, CD161, CLEC5B, HNKR-P1a, KLRB1, Killer cell lectin-like receptor subfamily B member 1, NKR-P1A, NKRP1A, Natural killer cell surface protein P1A |
| Species | Human |
| Expression Host | HEK293 Cells |
| Sequence | Gln67-Ser225 |
| Accession | Q12918 |
| Calculated Molecular Weight | 45.6 kDa |
| Observed Molecular Weight | 55-70 kDa |
| Tag | C-Fc |
| Bio-activity | Not validated for activity |
Properties
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
Killer cell lectin-like receptor subfamily B, member 1(KLRB1) is a single-pass type II membrane protein which contains 1 C-type lectin domain. KLRB1 plays an inhibitory role on natural killer (NK) cells cytotoxicity. Activation results in specific acid sphingomyelinase/SMPD1 stimulation with subsequent marked elevation of intracellular ceramide. Activation also leads to AKT1/PKB and RPS6KA1/RSK1 kinases stimulation as well as markedly enhanced T-cell proliferation induced by anti-CD3. It acts as a lectin that binds to the terminal carbohydrate Gal-alpha(1,3)Gal epitope as well as to the N-acetyllactosamine epitope. Binds also to CLEC2D/LLT1 as a ligand and inhibits NK cell-mediated cytotoxicity as well as interferon-gamma secretion in target cells.