Total Price: $0.00
Contact Information
Recombinant Human LDHA protein (His tag)
SKU: PDEH100141-100
To better serve you, we would like to discuss your specific requirement.
Please Contact Us for a quote.
Recombinant Human LDHA protein (His tag)
| SKU # | PDEH100141 |
| Expression Host | E. coli |
Description
| Synonyms | L-Lactate Dehydrogenase A Chain;LDH-A;Cell Proliferation-Inducing Gene 19 Protein;LDH Muscle Subunit;LDH-M;Renal Carcinoma Antigen NY-REN-59;LDHA |
| Species | Human |
| Expression_host | E.coli |
| Sequence | Ala 2-Phe 332 |
| Accession | P00338 |
| Mol_Mass | 36.3 kDa |
| AP_Mol_Mass | 39 kDa |
| Tag | N-His |
| Bio_Activity | Not validated for activity |
Properties
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin level | Please contact us for more information. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis |
Background
L-Lactate Dehydrogenase A Chain (LDHA) is an enzyme that catalyzes the conversion of L-lactate and NAD+ to pyruvate and NADH in the final step of anaerobic glycolysis. LDHA contains an N-terminal coenzyme binding region, a central catalytic site, and at least nine utilized Lys acetylation and two Tyr phosphorylation sites. LDHA belongs to the lactate dehydrogenase family, expressed predominantly in muscle tissue. LDHA mutations have been linked to exertional myoglobinuria.