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Recombinant Human Legumain/LGMN Protein (His Tag)
SKU: PKSH032687-50
Recombinant Human Legumain/LGMN Protein (His Tag)
| SKU # | PKSH032687 |
| Expression Host | HEK293 Cells |
Description
| Synonyms | Asparaginyl Endopeptidase, LGMN, Legumain, PRSC1, Protease Cysteine 1 |
| Species | Human |
| Expression Host | HEK293 Cells |
| Sequence | Ile18-Tyr433 |
| Accession | AAH03061.1 |
| Calculated Molecular Weight | 48.7 kDa |
| Observed Molecular Weight | 56 kDa |
| Tag | C-His |
| Bio-activity | Not validated for activity |
Properties
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles. |
| Shipping | This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C. |
| Formulation | Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, 10% Glycerol, pH 8.0. |
| Reconstitution | Not Applicable |
Background
Legumain is a lysosomal cysteine protease which is a member of the peptidase C13 family. Though it is found in many tissues, it is highly expressed in the kidney, heart, and placenta. Legumain has a strict specificity for hydrolysis of asparaginyl bonds and can also cleave aspartyl bonds slowly, especially under acidic conditions. Over-expression Legumain in tumors is significant for invasion and metastasis. In addition, Legumain may be involved in the processing of proteins for MHC class II antigen presentation in the lysosomal/endosomal system and negative regulation of neuron apoptosis.