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Recombinant Human LYPLA1 Protein (His Tag)
SKU: PKSH032043-50
Recombinant Human LYPLA1 Protein (His Tag)
| SKU # | PKSH032043 |
| Expression Host | E.coli |
Description
| Synonyms | APT-1, APT1, Acyl-Protein Thioesterase 1, LPL-I, LPL1, LYPLA1, LysoPLA I, Lysophospholipase 1, Lysophospholipase I, hAPT1 |
| Species | Human |
| Expression Host | E.coli |
| Sequence | Met 1-Asp230 |
| Accession | O75608 |
| Calculated Molecular Weight | 26.8 kDa |
| Observed Molecular Weight | 25 kDa |
| Tag | N-His |
| Bio-activity | Not validated for activity |
Properties
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles. |
| Shipping | This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C. |
| Formulation | Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 100mM NaCl, 1mM DTT, 10% Glycerol, pH 8.0. |
| Reconstitution | Not Applicable |
Background
Acyl-Protein Thioesterase 1 (APT-1) is lysophospholipase that belongs to the AB hydrolase 2 family. Lysophospholipases are enzymes that act on biological membranes to regulate the multifunctional lysophospholipids. APT-1 performs on biological membranes to regulate the multifunctional lysophospholipids. It hydrolyzes lysophosphatidylcholine in both monomeric and micellar forms. It hydrolyzes fatty acids from S-acylated cysteine residues in proteins such as trimeric G alpha proteins or HRAS; in addition, it also has depalmitoylating activity and low lysophospholipase activity.