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Recombinant Human MGMT Protein (His Tag)
SKU: PKSH032031-50
Recombinant Human MGMT Protein (His Tag)
| SKU # | PKSH032031 |
| Expression Host | E.coli |
Description
| Synonyms | 6-O-methylguanine-DNAmethyltransferase, MGMT, Methylated-DNA--protein-cysteine methyltransferase, O-6-methylguanine-DNA-alkyltransferase |
| Species | Human |
| Expression Host | E.coli |
| Sequence | Met 1-Asn207 |
| Accession | P16455 |
| Calculated Molecular Weight | 23.8 kDa |
| Observed Molecular Weight | 25 kDa |
| Tag | N-His |
| Bio-activity | Not validated for activity |
Properties
| Purity | > 90 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles. |
| Shipping | This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C. |
| Formulation | Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl,1mM DTT,1mM EDTA,500mM NaCl,0.1%Trition X-100,pH 8.0. |
| Reconstitution | Not Applicable |
Background
MGMT belongs to the family of transferases, specifically those transferring one-carbon group methyltransferases. MGMT involved in the cellular defense against the biological effects of O6-methylguanine in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. MGMT catalyzes the chemical reaction: DNA (containing 6-O-methylguanine) and proteinL-cysteine into DNA (without 6-O-methylguanine) and protein S-methyl-L-cysteine.