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Recombinant Human MIF Protein
SKU: PKSH032727-100
Recombinant Human MIF Protein
| SKU # | PKSH032727 |
| Expression Host | E.coli |
Description
| Synonyms | GLIF, Glycosylation-inhibiting factor, L-dopachrome tautomerase, MIF, MMIF, Macrophage migration inhibitory factor, Phenylpyruvate tautomerase |
| Species | Human |
| Expression Host | E.coli |
| Sequence | Met 1-Ala115 |
| Accession | P14174 |
| Calculated Molecular Weight | 13.3 kDa |
| Observed Molecular Weight | 15 kDa |
| Tag | C-His |
| Bio-activity | Not validated for activity |
Properties
| Purity | > 98 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 0.1 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from sterile PBS,pH 8.0. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
Human MIF is a 12.5 kDa, 115 amino acid (aa) nonglycosylated polypeptide that is synthesized without asignal sequence .Secretion occurs nonclassically via an ABCA1 transporter.Pro-inflammatory cytokine.Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites ofinflammation suggests a role as mediator in regulating the function of acrophages in host defense.Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase anddopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clearwhether the tautomerase activity has any physiological relevance, and whether it is important for cytokineactivity.