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Recombinant Human N-Glycosylase/OGG1 Protein (GST Tag)
SKU: PKSH032809-50
Recombinant Human N-Glycosylase/OGG1 Protein (GST Tag)
| SKU # | PKSH032809 |
| Expression Host | E.coli |
Description
| Synonyms | 8-Oxoguanine DNA Glycosylase, AP Lyase, DNA-(Apurinic or Apyrimidinic Site) Lyase, MMH, MUTM, N-Glycosylase/DNA Lyase, OGG1, OGH1 |
| Species | Human |
| Expression Host | E.coli |
| Sequence | Met 1-Gly345 |
| Accession | AAH00657.1 |
| Calculated Molecular Weight | 38.8 kDa |
| Observed Molecular Weight | 38 kDa |
| Tag | None |
| Bio-activity | Not validated for activity |
Properties
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles. |
| Shipping | This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C. |
| Formulation | Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, 1mM EDTA, pH 8.5. |
| Reconstitution | Not Applicable |
Background
Human N-Glycosylase/DNA Lyase(OOG1) is a DNA repair enzyme, which belongs to the type-1 OGG1 family. OOG1 incises DNA at 8-oxoG residues, and excises 7,8-dihydro-8-oxoguanine and 2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine (FAPY) from damage DNA. It has a β-lyase activity that nicks DNA 3’ to the lesion. OOG1 together with APEX1 is recruited to nuclear speckles in UVA-irradiated cells. The OGG1 gene mutations may be caused Renal cell carcinoma.