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Recombinant Human PDGFRa/CD140a Protein (His Tag)(Active)
SKU: PKSH031528-100
Recombinant Human PDGFRa/CD140a Protein (His Tag)(Active)
| SKU # | PKSH031528 |
| Expression Host | HEK293 Cells |
Description
| Synonyms | Alpha platelet-derived growth factor receptor, CD140 antigen-like family member A, CD140A, PDGFR-2, PDGFR-alpha, PDGFR2, Platelet-derived growth factor alpha receptor, Platelet-derived growth factor recepto, Platelet-derived growth factor receptor alpha, RHEPDGFRA |
| Species | Human |
| Expression Host | HEK293 Cells |
| Sequence | Met 1-Glu 524 |
| Accession | NP_006197.1 |
| Calculated Molecular Weight | 57.7 kDa |
| Observed Molecular Weight | 90-100 kDa |
| Tag | C-His |
| Bio-activity | Measured by its ability to bind human PDGFC-Fc in functional ELISA. |
Properties
| Purity | > 97 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from sterile PBS, pH 7.4 Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
PDGFRA; also known as CD140a; together with the structurally homolog protein PDGFRB (CD140b); are cell surface receptors for members of the platelet-derived growth factor family. They are members of the class III subfamily of receptor tyrosine kinase (RTKs) with the similar structure characteristics of five immunoglobulin-like domains in their extracellular region and a split kinase domain in their intracellular region. PDGFRA is expressed in oligodendrocyte progenitor cells and mesothelial cell; and binds all three ligand isoforms PDGF-AA; PDGF-BB and PDGF-AB with high affinity; whereas PDGFRB dose not bind PDGF-AA. PDGFRA plays an essential role in regulating proliferation; chemotaxis and migration of mesangial cells. Recent studies have indicated that PDGFRA acts as a critical mediator of signaling in testis organogenesis and Leydig cell differentiation; and in addition; particularly important for kidney development. Additionally; PDGFRA is involved in tumor angiogenesis and maintenance of the tumor microenvironment and has been implicated in development and metastasis of Hepatocellular carcinoma (HCC). PDGFRA may represent a potential therapeutic target in thymic tumours. PDGFRA gene amplification rather than gene mutation may be the underlying genetic mechanism driving PDGFRA overexpression in a portion of gliomas.