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Recombinant Human PFKFB1 Protein (His Tag)
SKU: PKSH032459-50
Recombinant Human PFKFB1 Protein (His Tag)
| SKU # | PKSH032459 |
| Expression Host | HEK293 Cells |
Description
| Synonyms | 6-P2ase liver isozyme, 6-bisphosphatase, 6-bisphosphatase 1, 6-phosphofructo-2-kinase/fructose-2, 6PF-2-K/Fru-2, F6PK, Fructose-2, PFKFB1, PFRX |
| Species | Human |
| Expression Host | HEK293 Cells |
| Sequence | Ser2-Tyr471 |
| Accession | P16118 |
| Calculated Molecular Weight | 55.6 kDa |
| Observed Molecular Weight | 60 kDa |
| Tag | C-His |
| Bio-activity | Not validated for activity |
Properties
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles. |
| Shipping | This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C. |
| Formulation | Supplied as a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, 5% Trehalose, 1mM EDTA, pH 7.8. |
| Reconstitution | Not Applicable |
Background
6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 1 is an enzyme that in humans is encoded by the PFKFB1 gene. The enzyme forms a homodimer that catalyzes both the synthesis and degradation of fructose-2,6-biphosphate using independent catalytic domains. It belongs to the phosphoglycerate mutase family. Fructose-2,6-biphosphate is an activator of the glycolysis pathway and an inhibitor of the gluconeogenesis pathway. Consequently, regulating fructose-2,6-biphosphate levels through the activity of this enzyme is thought to regulate glucose homeostasis.