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Recombinant Human PLA2G7/Lp-PLA2 Protein (His Tag)(Active)– MSE Supplies LLC

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Recombinant Human PLA2G7/Lp-PLA2 Protein (His Tag)(Active)

SKU: PKSH031390-50

  • $ 64895
  • Save $ 7300



Recombinant Human PLA2G7/Lp-PLA2 Protein (His Tag)(Active)

 

SKU # PKSH031390
Expression Host HEK293 Cells

 

 

Description

Synonyms 1-alkyl-2-acetylglycerophosphocholine esterase, 2-acetyl-1-alkylglycerophosphocholine esterase, EC 3.1.1, EC 3.1.1.47, Group-VIIA phospholipase A2, LDL-PLA(2), LDL-PLA2, LDL-associated phospholipase A2, Lp, LpPLA2, gVIIA-PLA2, lipoprotein-associated phospholipase A2
Species Human
Expression Host HEK293 Cells
Sequence Met 1-Asn 441
Accession Q13093-1
Calculated Molecular Weight 49.2 kDa
Observed Molecular Weight 50-55 kDa
Tag C-His
Bio-activity Measured by its ability to cleave a colorimetric peptide substrate, 1O-hexadecyl-2-deoxy-2-thio Sacetylsnglyceryl-3-phosphoryl choline (2-Thio-PAF), in the presence of 5, 5’Dithiobis(2-nitrobenzoic acid) (DTNB). The specific activity is > 5000 pmoles/min/μg.
  

 

Properties

Purity > 88 % as determined by reducing SDS-PAGE.
Endotoxin < 1.0 EU per μg of the protein as determined by the LAL method.
Storage Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping This product is provided as lyophilized powder which is shipped with ice packs.
Formulation Lyophilized from sterile 50mM NaAc, 150mM NaCl, 10% glycerol, pH 5.0
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution Please refer to the printed manual for detailed information.



Background

Platelet-activating factor acetylhydrolase; also known as 1-alkyl-2-acetylglycerophosphocholine esterase; 2-acetyl-1-alkylglycero-phosphocholine esterase; Group-VIIA phospholipase A2; LDL-associated phospholipase A2; PAF 2-acylhydrolase; PLA2G7 and PAFAH; is secreted protein which belongs to theAB hydrolase superfamily and Lipase family. PLA2G7 / PAFAH modulates the action of platelet-activating factor (PAF) by hydrolyzing the sn-2 ester bond to yield the biologically inactive lyso-PAF. It has a specificity for substrates with a short residue at the sn-2 position. It is inactive against long-chain phospholipids. PLA2G7 / PAFAH is a potent pro- and anti-inflammatory molecule that has been implicated in multiple inflammatory disease processes; including cardiovascular disease. PLA2G7 also represents an important; potentially functional candidate in the pathophysiology of coronary artery disease (CAD). Defects in PLA2G7 are the cause of platelet-activating factor acetylhydrolase deficiency (PLA2G7 deficiency). It is a trait which is present in 27% of Japanese. It could have a significant physiologic effect in the presence of inflammatory bodily responses.