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Recombinant Human PPCDC Protein (His Tag)
SKU: PKSH032896-50
Recombinant Human PPCDC Protein (His Tag)
| SKU # | PKSH032896 |
| Expression Host | E.coli |
Description
| Synonyms | COAC, PPC-DC, PPCDC, Phosphopantothenoylcysteine Decarboxylase |
| Species | Human |
| Expression Host | E.coli |
| Sequence | Met 1-Ser204 |
| Accession | Q96CD2 |
| Calculated Molecular Weight | 24.6 kDa |
| Observed Molecular Weight | 27 kDa |
| Tag | N-His |
| Bio-activity | Not validated for activity |
Properties
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles. |
| Shipping | This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C. |
| Formulation | Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 50mM NaCl, 1mM DTT, 10% Glycerol, pH 8.0. |
| Reconstitution | Not Applicable |
Background
Phosphopantothenoylcysteine Decarboxylase (PPC-DC) is an essential enzyme in the biosynthesis ofCoenzyme A and catalyzes the decarboxylation of PPC to Phosphopantetheine. PPC-DC catalyzes the decarboxylation of the Cysteine moiety of 4-Phosphopantothenoylcysteine (PPC) to form 4-Phosphopantetheine (PPantSH), this reaction forms part of the biosynthesis of Coenzyme A. The enzyme is a member of the larger family of Cysteine Decarboxylases including the Lantibiotic-Biosynthesizing enzymes EpiD and MrsD, all of which use a tightly bound Flavin cofactor to oxidize the Thiol moiety of the substrate to a Thioaldehyde.