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Recombinant Human PPP1R14A Protein (His Tag)
SKU: PKSH032967-50
Recombinant Human PPP1R14A Protein (His Tag)
| SKU # | PKSH032967 |
| Expression Host | E.coli |
Description
| Synonyms | 17 kDa PKC-Potentiated Inhibitory Protein of PP1, CPI-17, CPI17, PPP1INL, PPP1R14A, Protein Kinase C-Potentiated Inhibitor Protein of 17 kDa, Protein Phosphatase 1 Regulatory Subunit 14A |
| Species | Human |
| Expression Host | E.coli |
| Sequence | Met 1-Pro147 |
| Accession | Q96A00 |
| Calculated Molecular Weight | 17.8 kDa |
| Observed Molecular Weight | 23-26 kDa |
| Tag | C-His |
| Bio-activity | Not validated for activity |
Properties
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles. |
| Shipping | This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C. |
| Formulation | Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 0.2mM EDTA, 1mM DTT, 10% Glycerol, pH 8.0. |
| Reconstitution | Not Applicable |
Background
Protein Phosphatase 1 Regulatory Subunit 14A (PPP1R14A) belongs to the PP1 inhibitor family. PPP1R14A is mapped to chromosome 19q13.13-q13.2. PPP1R14A binds directly to protein kinase C and casein kinase I. Meantime; PPP1R14A is a phosphorylation-dependent inhibitor of smooth muscle myosin phosphatase. PPP1R14A is the inhibitor of PPP1CA. When phosphorylated; PPP1R14A has over 1000-fold higher inhibitory activity; creating a molecular switch for regulating the phosphorylation status of PPP1CA substrates and smooth muscle contraction. In addition; inhibition of PPP1R14A also enhances contraction of smooth muscle in the absence of increment of intracellular Ca2+ concentration.