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Recombinant Human PPP1R1A Protein (His Tag)
SKU: PKSH032968-50
Recombinant Human PPP1R1A Protein (His Tag)
| SKU # | PKSH032968 |
| Expression Host | E.coli |
Description
| Synonyms | I-1, IPP-1, IPP1, PPP1R1A, Protein Phosphatase 1 Regulatory Subunit 1A, Protein Phosphatase Inhibitor 1 |
| Species | Human |
| Expression Host | E.coli |
| Sequence | Met 1-Val171 |
| Accession | AAH22470.1 |
| Calculated Molecular Weight | 20.0 kDa |
| Observed Molecular Weight | 18 kDa |
| Tag | C-His |
| Bio-activity | Not validated for activity |
Properties
| Purity | > 90 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles. |
| Shipping | This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C. |
| Formulation | Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, 1mM DTT, 50% Glycerol, pH 8.5. |
| Reconstitution | Not Applicable |
Background
Protein Phosphatase 1 Regulatory Subunit 1A (PPP1R1A) is an inhibitor of protein-phosphatase 1. PPP1R1A is a cellular regulator of eIF2 alpha phosphorylation. In hormonal control of glycogen metabolism, IPP-1 protein plays important function. Hormones can elevate intracellular cAMP level and elevate IPP-1 activity. PPP1R1A activation caused cAMP increase , cAMP control over proteins that are not directly phosphorylated by PKA following a rise in intracellular calcium. IPP-1 is inactivated by calcineurin (PP2B). Multiple domains in IPP-1 target cellular PP1 complexes.