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Recombinant Human PRKG1 Protein (His Tag)
SKU: PKSH032242-50
Recombinant Human PRKG1 Protein (His Tag)
| SKU # | PKSH032242 |
| Expression Host | HEK293 Cells |
Description
| Synonyms | PRKG1, PRKG1B, PRKGR1A, PRKGR1B, cGK 1, cGK1, cGKI, cGMP-Dependent Protein Kinase 1, cGMP-Dependent Protein Kinase I |
| Species | Human |
| Expression Host | HEK293 Cells |
| Sequence | Gly2-Pro686 |
| Accession | Q13976-2 |
| Calculated Molecular Weight | 78.8 kDa |
| Observed Molecular Weight | 75 kDa |
| Tag | C-His |
| Bio-activity | Not validated for activity |
Properties
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles. |
| Shipping | This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C. |
| Formulation | Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 6% Sucrose, 4% Mannitol, 0.05% Tween 80, pH8.0. |
| Reconstitution | Not Applicable |
Background
cGMP-Dependent Protein Kinase 1 (PRKG1) belongs to the protein kinase superfamily and AGC Ser/Thr protein kinase family. PRKG1 contains one AGC-kinase C-terminal domain, two cyclic nucleotide-binding domains, and one protein kinase domain. PRKG1 is mainly expressed in the lung and placenta. PRKG1 acts as a key mediator of the nitric oxide (NO)/cGMP signaling pathway. PRKG1 can phosphorylate many proteins that regulate platelet activation and adhesion, smooth muscle contraction, cardiac function, gene expression, feedback of the NO-signaling pathway, and other processes involved in several aspects of the CNS like axon guidance, hippocampal and cerebellar learning, circadian rhythm, and nociception.