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Recombinant Human PRV1/CD177 Protein (His Tag)
SKU: PKSH032980-50
Recombinant Human PRV1/CD177 Protein (His Tag)
| SKU # | PKSH032980 |
| Expression Host | HEK293 Cells |
Description
| Synonyms | CD177, CD177 Antigen, HNA-2a, Human Neutrophil Alloantigen 2a, NB1, NB1 GP, NB1 Glycoprotein, PRV-1, PRV1, Polycythemia Rubra Vera Protein 1 |
| Species | Human |
| Expression Host | HEK293 Cells |
| Sequence | Leu22-Gly407 |
| Accession | AAH29167.1 |
| Calculated Molecular Weight | 42.3 kDa |
| Observed Molecular Weight | 55 kDa |
| Tag | C-His |
| Bio-activity | Not validated for activity |
Properties
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.2. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
CD177 is polymorphic and has at least two alleles: PRV1 and NB1. Human PRV1 is a Glycosyl-Phosphatidylinositol (GPI)-linked cell surface glycoprotein that belongs to the uPAR/CD59/Ly6 family of receptors. PRV1 is expressed by neutrophils and neutrophil precursors,and changes in expression serve as diagnostic markers for myeloproliferative disorders such as polycythemia vera and essential thrombocythemia. PRV1 may also be expressed by Erythroblasts, B cells, and Monocytes. NB1, a Glycosyl-Phosphatidylinositol (GPI)-linked cell surface glycoprotein, was first described in a case of neonatal alloimmune neutropenia. It is reported that CD177 functions as a novel heterophilic binding partner that engages PECAM-1 in membrane-proximal IgD6.