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Recombinant Human/Rhesus HER4/ErbB4 Protein (Fc Tag)(Active)
SKU: PKSH031650-100
Recombinant Human/Rhesus HER4/ErbB4 Protein (Fc Tag)(Active)
| SKU # | PKSH031650 |
| Expression Host | HEK293 Cells |
Description
| Synonyms | ERBB4, HER4, Proto-oncogene-like protein c-ErbB-4, Receptor tyrosine-protein kinase erbB-4, Tyrosine kinase-type cell surface receptor HER4, p180erbB4 |
| Species | Human |
| Expression Host | HEK293 Cells |
| Sequence | Met 1-Arg649 |
| Accession | NP_005226.1 |
| Calculated Molecular Weight | 96.6 kDa |
| Observed Molecular Weight | 117 kDa |
| Tag | C-hFc |
| Bio-activity | 1. Measured by its ability to bind biotinylated human Fc-NRG1 (isoform Beta1) in a functional ELISA. 2. Measured by its ability to bind biotinylated human NRG1 (isoform Beta1) in a functional ELISA. 3. Measured by its ability to bind biotinylated human NRG1 (aa 2-246)-Fc in a functional ELISA. |
Properties
| Purity | > 85 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from sterile PBS, pH7.4 Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
ERBB4 is a single-pass type I membrane protein with multiple cysteine rich domains; a transmembrane domain; a tyrosine kinase domain; a phosphotidylinositol-3 kinase binding site and a PDZ domain binding motif. ERBB4 is expressed at highest levels in brain; heart; kidney; in addition to skeletal muscle; parathyroid; cerebellum; pituitary; spleen; testis and breast. And lower levels in thymus; lung; salivary gland; and pancreas. It specifically binds to and is activated by neuregulins; NRG-2; NRG-3; heparin-binding EGF-like growth factor; betacellulin and NTAK. ERBB4 also can be activated by other factors and induces a variety of cellular responses including mitogenesis and differentiation. ERBB4 regulates development of the heart; the central nervous system and the mammary gland; gene transcription; cell proliferation; differentiation; migration and apoptosis. It is required for normal cardiac muscle differentiation during embryonic development; and for postnatal cardiomyocyte proliferation. ERBB4 also play a role on the normal development of the embryonic central nervous system; especially for normal neural crest cell migration and normal axon guidance. It is required for mammary gland differentiation; induction of milk proteins and lactation.