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Recombinant Human Ribonuclease 3/RNASE3 Protein (His Tag)
SKU: PKSH033002-50
Recombinant Human Ribonuclease 3/RNASE3 Protein (His Tag)
| SKU # | PKSH033002 |
| Expression Host | HEK293 Cells |
Description
| Synonyms | ECP, Eosinophil Cationic Protein, RNASE3, RNS3, RNase 3, Ribonuclease 3 |
| Species | Human |
| Expression Host | HEK293 Cells |
| Sequence | Arg28-Ile160 |
| Accession | AAH96060.1 |
| Calculated Molecular Weight | 16.6 kDa |
| Observed Molecular Weight | 25-35 kDa |
| Tag | C-His |
| Bio-activity | Not validated for activity |
Properties
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles. |
| Shipping | This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C. |
| Formulation | Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, 1mMDTT, 10% Glycerol, pH 7.5. |
| Reconstitution | Not Applicable |
Background
Ribonuclease 3 (RNASE3) is a basic protein that is localized to the eosinophil primary matrix and belongs to the pancreatic ribonuclease family. RNASE3 is released during degranulation of eosinophils. RNASE3 possesses a wide variety of biological activities. RNASE3 interacts with bacterial lipopolysaccharide (LPS) and lipoteichoic acid (LTA). RNASE3 exhibits antibacterial activity, including cytoplasmic membrane depolarization of preferentially Gram-negative, but also Gram-positive strains. It promotes E. coli outer membrane detachment, alteration of the overall cell shape and partial loss of cell content.