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Recombinant Human SHISA3 Protein (Fc Tag)
SKU: PKSH030300-100
Recombinant Human SHISA3 Protein (Fc Tag)
| SKU # | PKSH030300 |
| Expression Host | HEK293 Cells |
Description
| Synonyms | hShisa3 |
| Species | Human |
| Expression Host | HEK293 Cells |
| Sequence | Met 1-Pro 98 |
| Accession | AAI27691.1 |
| Calculated Molecular Weight | 34.9 kDa |
| Tag | C-mFc |
| Bio-activity | Not validated for activity |
| Bio-activity | Measured by its binding ability in a functional ELISA. Immobilized human CD32a-Fc at 10 μg/ml (100 μl/well) can bind biotinylated human IgG1 with a linear range of 0.625-10 μg/ml. |
Properties
| Purity | > 90 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from sterile PBS, pH 7.4 Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
SHISA3 encodes a single-transmembrane protein which is one of nine members of a family of transmembrane adaptors that modulate both WNT and FGF signaling by blocking the maturation and transport of their receptors to the cell surface. Members of this family contain an N-terminal cysteine-rich domain with a distinct cysteine pattern, a single transmembrane domain, and a C-terminal proline-rich, low complexity region. The encoded protein acts as a tumor suppressor by accelerating beta-catenin degradation.