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Recombinant Human SIGLEC5 Protein
SKU: PKSH030999-100
Recombinant Human SIGLEC5 Protein
| SKU # | PKSH030999 |
| Expression Host | HEK293 Cells |
Description
| Synonyms | CD170, CD33 antigen-like 2, CD33L2, OB-BP2, OBBP, OBBP2, Obesity-binding protein 2, SIGLEC-5, SIGLEC5, Sialic acid-binding Ig-like lectin 5, Siglec-5 |
| Species | Human |
| Expression Host | HEK293 Cells |
| Sequence | Met 1-Thr 434 |
| Accession | O15389 |
| Calculated Molecular Weight | 47.2 kDa |
| Observed Molecular Weight | 67 kDa |
| Tag | None |
| Bio-activity | Not validated for activity |
Properties
| Purity | > 85 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from sterile PBS, pH 7.4 Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
SIGLEC5 contains 2 Ig-like C2-type (immunoglobulin-like) domains and 1 Ig-like V-type (immunoglobulin-like) domain. It belongs to the immunoglobulin superfamily and SIGLEC (sialic acid binding Ig-like lectin) family. SIGLEC5 is expressed by monocytic/myeloid lineage cells. It is found at high levels in peripheral blood leukocytes; spleen; bone marrow and at lower levels in lymph node; lung; appendix; placenta; pancreas and thymus. It is also expressed by monocytes and neutrophils but absent from leukemic cell lines representing early stages of myelomonocytic differentiation. SIGLEC5 is a putative adhesion molecule that mediates sialic-acid dependent binding to cells. It binds equally to alpha-2;3-linked and alpha-2;6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface.