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Recombinant Human SLAMF7/CD319 Protein (His Tag)(Active)
SKU: PKSH031026-100
Recombinant Human SLAMF7/CD319 Protein (His Tag)(Active)
| SKU # | PKSH031026 |
| Expression Host | HEK293 Cells |
Description
| Synonyms | CD2 Subset 1, CD2-Like Receptor-Activating Cytotoxic Cells, CD319, CRACC, CS1, Membrane Protein FOAP-12, Novel Ly9, Protein 19A, SLAM Family Member 7, SLAM7, SLAMF7 |
| Species | Human |
| Expression Host | HEK293 Cells |
| Sequence | Met 1-Met 226 |
| Accession | NP_067004.3 |
| Calculated Molecular Weight | 23.8 kDa |
| Tag | C-His |
| Bio-activity | Measured by its ability to bind biotinylated human SH2D1A-His in a functional ELISA. |
| Bio-activity | Measured in antiviral assays using WISH human amnion cells infected with vesicular stomatitis virus (VSV). The EC50 for this effect is typically 0.1-0.5 ng/mL. |
Properties
| Purity | > 93 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from sterile PBS, pH 7.4 Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
SLAM family member 7 (SLAMF7), also known as CRACC, CD319, CD2-like receptor-activating cytotoxic cells, and CS1, is a single-pass type I membrane protein and a member of the CD2 family of cell surface receptors. SLAMF7 is expressed in NK cells, activated B-cells, NK-cell line but not in promyelocytic, B-cell lines, or T-cell lines. Although the cytoplasmic domain of CS1 contains immunoreceptor tyrosine-based switch motifs (ITSM), which enables to recruite signaling lymphocyte activation molecule (SLAM)-associated protein (SAP/SH2D1A), it activates NK cells in the absence of a functional SAP. CS1 is a self ligand and homophilic interaction of CS1 regulates NK cell cytolytic activity. CRACC positively regulated natural killer cell functions by a mechanism dependent on the adaptor EAT-2 but not the related adaptor SAP. However, in the absence of EAT-2, CRACC potently inhibited natural killer cell function. It was also inhibitory in T cells, which are typically devoid of EAT-2. Thus, CRACC can exert activating or inhibitory influences on cells of the immune system depending on cellular context and the availability of effector proteins.