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Recombinant Human Sonic Hedgehog/SHH Protein (aa 1-197, His Tag)(Active)
SKU: PKSH031645-100
Recombinant Human Sonic Hedgehog/SHH Protein (aa 1-197, His Tag)(Active)
| SKU # | PKSH031645 |
| Expression Host | HEK293 Cells |
Description
| Synonyms | HHG-1, SHH, Sonic Hedgehog Protein |
| Species | Human |
| Expression Host | HEK293 Cells |
| Sequence | Met 1-Gly 197 |
| Accession | Q15465 |
| Calculated Molecular Weight | 21 kDa |
| Observed Molecular Weight | 24 kDa |
| Tag | C-His |
| Bio-activity | Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells. The ED50 for this effect is typically 2-10 μg/mL. |
Properties
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from sterile PBS, pH 7.4 Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
Sonic HedgeHog; also known as sonic hedgehog protein; belongs to the hedgehog family. It cannot be detected in adult tissues while can be found in fetal intestine; liver; lung; and kidney. Sonic HedgeHog is a protein that is vital in guding the early embryo. It has been associated as the major inductive signal in patterning of the ventral neural tube; the anterior-posterior limb axis; and the ventral somites. Sonic HedgeHog intercellular signal is essential for a various patterning events during development: signal produced by the notochord that induces ventral cell fate in the neural tube and somites; and the polarizing signal for patterning of the anterior-posterior axis of the developing limb bud. Sonic HedgeHog binds to the patched receptor; which functions in association with smoothened; to activate the transcription of target genes. In the absence of sonic HedgeHog; patched receptor represses the constitutive signaling activity of smoothened. Sonic HedgeHog also regulates another factor; the gli oncogene. Defects in sonic hedgehog can cause microphthalmia isolated with coloboma type 5; triphalangeal thumb-polysyndactyly syndrome and holoprosencephaly type 3.