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Recombinant Human SUMO1 Protein (His Tag)
SKU: PKSH033311-50
Recombinant Human SUMO1 Protein (His Tag)
| SKU # | PKSH033311 |
| Expression Host | E.coli |
Description
| Synonyms | DAP1, GAP-Modifying Protein 1, GMP1, OFC10, SENP2, SMT3, SMT3 Homolog 3, SMT3C, SMT3H3, SUMO-1, SUMO1, Sentrin, Small Ubiquitin-Related Modifier 1, Smt3C, UBL1, Ubiquitin-Homology Domain Protein PIC1, Ubiquitin-Like Protein, Ubiquitin-Like Protein SMT3C |
| Species | Human |
| Expression Host | E.coli |
| Sequence | Met 1-Val101 |
| Accession | AAH66306 |
| Calculated Molecular Weight | 13.7 kDa |
| Observed Molecular Weight | 17-19 kDa |
| Tag | N-His |
| Bio-activity | Not validated for activity |
Properties
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from a 0.2 μm filtered solution of 50mM Tris-HCl, 100mM NaCl, 1mM DTT, pH 8.5 . Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
Small Ubiquitin-Related Modifier 1 (SUMO1) is an Ubiquitin-like protein that belongs to the ubiquitin family with SUMO subfamily. It is a family of small, related proteins that can be enzymatically attached to a target protein by a post-translational modification process termed sumoylation. SUMO1 functions in a manner similar to ubiquitin in that it is bound to target proteins as part of a post-translational modification system. This post-translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. SUMO1 is involved in a variety of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability. SUMO1 is not active until the last four amino acids of the carboxy-terminus are cleaved off. Polymeric SUMO1 chains are also susceptible to polyubiquitination which functions as a signal for proteasomal degradation of modified proteins and may also regulate a network of genes involved in palate development.