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Recombinant Human TIMP-1 Protein (His tag)
SKU: PDMH100115-100
Recombinant Human TIMP-1 Protein (His tag)
| SKU # | PDMH100115 |
| Expression Host | HEK293 Cells |
Description
| Synonyms | CLGI, Collagenase Inhibitor, EPA, EPO, Erythroid-Potentiating Activity, Fibroblast collagenase Inhibitor, HCI, Metalloproteinase Inhibitor 1, TIMP, TIMP-1, TIMP1, Tissue Inhibitor of Metalloproteinases 1 |
| Species | Human |
| Expression Host | HEK293 Cells |
| Sequence | Met1-Ala207 |
| Accession | P01033 |
| Calculated Molecular Weight | 22.7 kDa |
| Observed Molecular Weight | 30 kDa |
| Tag | C-His |
| Bio-activity | Not validated for activity |
Properties
| Purity | > 95% as determined by reducing SDS-PAGE. |
| Endotoxin | Please contact us for more information. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from sterile PBS, pH 7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis. |
Background
Tissue Inhibitor of Metalloproteinases 1 (TIMP-1) is a member of TIMP family. The homologous proteins of TIMPs regulate the activity of matrix metalloproteinases (MMPs), including inhibition of active MMPs, proMMP activation, cell growth promotion, matrix binding, inhibition of angiogenesis and the induction of apoptosis. Timp-1 complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. It also mediates erythropoiesis in vitro, but, unlike IL-3, it is species-specific, stimulating the growth and differentiation of only human and murine erythroid progenitors. It is known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-11, MMP-12, MMP-13, and MMP-16, without MMP-14.