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Recombinant Human TRAIL R2/TNFRSF10B Protein (Fc & His Tag)
SKU: PKSH033127-50
Recombinant Human TRAIL R2/TNFRSF10B Protein (Fc & His Tag)
| SKU # | PKSH033127 |
| Expression Host | HEK293 Cells |
Description
| Synonyms | CD262, DR5, Death Receptor 5, KILLER, TNF-Related Apoptosis-Inducing Ligand Receptor 2, TNFRSF10B, TRAIL Receptor 2, TRAIL-R2, TRAILR2, TRICK2, Tumor Necrosis Factor Receptor Superfamily Member 10B, ZTNFR9 |
| Species | Human |
| Expression Host | HEK293 Cells |
| Sequence | Ile56-Glu182 |
| Accession | O14763 |
| Calculated Molecular Weight | 42.2 kDa |
| Observed Molecular Weight | 49 kDa |
| Tag | C-Fc-His |
| Bio-activity | Not validated for activity |
Properties
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
TNFRSF10B is a member of the TNF-receptor superfamily; and contains an intracellular death domain. This receptor can be activated by tumor necrosis factor-related apoptosis inducing ligand (TNFSF10/TRAIL/APO-2L); and transduces apoptosis signal. The adapter molecule FADD recruits caspase-8 to the activated receptor and is required for the apoptosis mediated by TNFRSF10B. TNFRSF10B is expressed in a number of cell types; and to particularly high levels in lymphocytes and spleen. This single-pass transmembrane protein contains two cysteine-rich repeat units in its extracellular region; followed by a transmembrane segment and a cytoplasmic tail containing a typical “death domain”. TNFRSF10B expression is regulated by the tumor suppressor p53. It is also indicated that the activation of NF-kappa-B can be promoted by TNFRSF10B.