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Recombinant Human Trefoil factor 3/TFF3 protein (GST,His tag)
SKU: PDEH100421-100
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Recombinant Human Trefoil factor 3/TFF3 protein (GST,His tag)
| SKU # | PDEH100421 |
| Expression Host | E. coli |
Description
| Synonyms | Trefoil factor 3;TFF3;Intestinal trefoil factor (hITF) |
| Species | Human |
| Expression_host | E.coli |
| Sequence | Glu 22- Phe 80 |
| Accession | Q07654 |
| Mol_Mass | 31.4 kDa |
| AP_Mol_Mass | 25 kDa |
| Tag | N-GST & C-His |
| Bio_Activity | Not validated for activity |
Propertie
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin level | Please contact us for more information. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis |
Background
Trefoil Factor 3 (TFF3) is one of three trefoil peptides secreted by epithelial cells that line mucus membranes. Trefoils contribute to protection and repair of the gastrointestinal tract. TFF3 is secreted by mucus-producing goblet cells in the large and small intestines and lower respiratory tract. It is mainly active as a disulfide-linked homodimer. The mature 59 aa, 6.5 kDa human TFF3 contains one trefoil structure formed by intramolecular disulfides and shows 76% aa identity with mouse TFF3.