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Recombinant Human TROP2/TACSTD2 Protein (His & Fc Tag)(Active)
SKU: PKSH031614-100
Recombinant Human TROP2/TACSTD2 Protein (His & Fc Tag)(Active)
| SKU # | PKSH031614 |
| Expression Host | HEK293 Cells |
Description
| Synonyms | EGP-1, EGP1, GA733-1, GA7331, GP50, M1S1, TACD2, TROP-2, TROP2 |
| Species | Human |
| Expression Host | HEK293 Cells |
| Sequence | Met 1-Thr 274 |
| Accession | NP_002344.2 |
| Calculated Molecular Weight | 55.7 kDa |
| Observed Molecular Weight | 65-75 kDa |
| Tag | C-His-Fc |
| Bio-activity | Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells. When cells are added to coated plates (10μg/ml, 100μl/well), approximately 55%-70% will adhere specifically. |
Properties
| Purity | > 98 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from sterile PBS, pH 7.4 Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
TROP-2, also referred to as tumor associated calcium signal transducer 2 (TACSTD2), GA733-1 or M1S1, is a cell surface glycoprotein highly expressed in a wide variety of epithelial cancers. In contrast, there is little or no expression of Trop-2 in adult somatic tissue. Because it is a cell surface protein that is selectively expressed in tumor cells, Trop-2 is a potential therapeutic target. The cytoplasmic tail of Trop-2 possesses potential serine and tyrosine phosphorylation sites and a phosphatidyl-inositol binding consensus sequence. Trop-2 transduces an intracellular calcium signal, are consistent with the hypothesis that it acts as a cell surface receptor and support a search for a physiological ligand. TROP2 encoding by an intronless gene was originally defined by the monoclonal antibody GA733, and is a member of a family of at least two type I membrane proteins. The other known member is GA733-2, also called EpCAM and TROP1. It has been suggested by studies that the GA733-1 gene was formed by the retroposition of the GA733-2 gene via an mRNA intermediate.