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Recombinant Human Troponin C/TNNC1 Protein
SKU: PKSH031309-100
Recombinant Human Troponin C/TNNC1 Protein
| SKU # | PKSH031309 |
| Expression Host | E.coli |
Description
| Synonyms | CMD1Z, CMH13, TN-C, TNC, TNNC |
| Species | Human |
| Expression Host | E.coli |
| Sequence | Met 1-Glu161 |
| Accession | NP_003271.1 |
| Calculated Molecular Weight | 18.4 kDa |
| Observed Molecular Weight | 20 kDa |
| Tag | None |
| Bio-activity | Not validated for activity |
Properties
| Purity | > 90 % as determined by reducing SDS-PAGE. |
| Endotoxin | Please contact us for more information. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from sterile 150 mM NaCl, 10 mM Na2HPO4, pH 7.5 Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
Troponin I, also known as TNNC1, is part of the troponin complex. This complex contains 3 subunits: troponin I (TnI), troponin T (TnT) and troponin C (TnC). Troponin I is the inhibitory subunit, blocking actin-myosin interactions and thereby mediating striated muscle relaxation. It binds to actin in thin myofilaments to hold the actin-tropomyosin complex in place. Because of it myosin cannot bind actin in relaxed muscle. When calcium binds to the Troponin C it causes conformational changes which lead to dislocation of troponin I and finally tropomyosin leaves the binding site for myosin on actin leading to contraction of muscle.