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Recombinant Human UBA5 Protein (His Tag)
SKU: PKSH033194-50
Recombinant Human UBA5 Protein (His Tag)
| SKU # | PKSH033194 |
| Expression Host | E.coli |
Description
| Synonyms | ThiFP1, UBA5, UBE1DC1, UFM1-activating enzyme, Ubiquitin-activating enzyme E1 domain-containing protein 1, Ubiquitin-like modifier-activating enzyme 5 |
| Species | Human |
| Expression Host | E.coli |
| Sequence | Met 1-Met404 |
| Accession | Q9GZZ9 |
| Calculated Molecular Weight | 47.0 kDa |
| Observed Molecular Weight | 14&40-60 kDa |
| Tag | N-His |
| Bio-activity | Not validated for activity |
Properties
| Purity | > 90 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles. |
| Shipping | This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C. |
| Formulation | Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 50mM NaCl, 1mM DTT, 10% Glycerol, pH8.0. |
| Reconstitution | Not Applicable |
Background
UBA5 is a member of the ubiquitin-activating E1 family and UBA5 subfamily. Ubiquitin and ubiquitin-like proteins are recognized as covalently conjugated to various cellular substrates by a three-step enzymatic pathway. The ubiquitin-activating enzyme (E1) has a vital role in the first step of ubiquitination pathway to activate ubiquitin or ubiquitin-like proteins. UBA5 activates ubiquitin-fold modifier 1, a ubiquitin-like post-translational modifier protein, via the formation of a high-energy thioester bond. UBA5 is located primarily in cytoplasm, while it generally localizes to the nucleus in presence of SUMO2.