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Recombinant Human UCHL1/PGP9.5 Protein (His Tag)
SKU: PKSH033175-50
Recombinant Human UCHL1/PGP9.5 Protein (His Tag)
| SKU # | PKSH033175 |
| Expression Host | E.coli |
Description
| Synonyms | HEL-117, NDGOA, Neuron Cytoplasmic Protein 9.5, PARK5, PGP 9.5, PGP9.5, UCH-L1, UCHL1, Ubiquitin Carboxyl-Terminal Hydrolase Isozyme L1, Ubiquitin Thioesterase L1, Uch-L1 |
| Species | Human |
| Expression Host | E.coli |
| Sequence | Met 1-Ala223 |
| Accession | P09936 |
| Calculated Molecular Weight | 25.9 kDa |
| Observed Molecular Weight | 26 kDa |
| Tag | C-His |
| Bio-activity | Not validated for activity |
Properties
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles. |
| Shipping | This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C. |
| Formulation | Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 250mM NaCl, 1mM DTT, 10% Glycerol, pH 7.5. |
| Reconstitution | Not Applicable |
Background
Ubiquitin Carboxyl-Terminal Hydrolase Isozyme L1 (UCHL1) belongs to the Peptidase C12 family. UCHL1 is specifically expressed in the neurons and in cells of the diffuse neuroendocrine system. UCHL1 is a component of the ubiquitin system, which has a fundamental role in regulating various biological activities. UCHL1 is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. UCHL1 also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer of UCHL1 may have ATP-independent ubiquitin ligase activity.