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Recombinant Human XPNPEP2 Protein (His Tag)(Active)
SKU: PKSH030976-50
Recombinant Human XPNPEP2 Protein (His Tag)(Active)
| SKU # | PKSH030976 |
| Expression Host | HEK293 Cells |
Description
| Synonyms | Aminoacylproline Aminopeptidase, Membrane-Bound APP, Membrane-Bound AmP, Membrane-Bound Aminopeptidase P, X-Pro Aminopeptidase 2, XPNPEP2, Xaa-Pro Aminopeptidase 2, mAmP |
| Species | Human |
| Expression Host | HEK293 Cells |
| Sequence | Met 1-Ala 650 |
| Accession | O43895 |
| Calculated Molecular Weight | 72.0 kDa |
| Tag | C-His |
| Bio-activity | Measured by its ability to cleave the fluorogenic peptide substrate, H-Lys(2-Aminobenzoyl)Pro-Pro-pNitroanilide(K(Abz)PP-pNA). The specific activity is > 300 pmoles/min/μg. |
Properties
| Purity | > 97 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from sterile PBS, pH 7.4 Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
Aminopeptidase P (APP) is a hydrolase specific for N-terminal imido bonds; which are common to several collagen degradation products; neuropeptides; vasoactive peptides; and cytokines. A membrane-bound and soluble form of this enzyme (XPNPEP2) have been identified as products of two separate genes. XPNPEP2; the X-linked gene that encodes membranous aminopeptidase P (APP); has been reported to associate with APP activity. The membrane aminopeptidase P (XPNPEP2) is largely limited in distribution to endothelia and brush border epithelia. APP and XPNPEP2 contain homologous blocks of sequence common to members of the "pita bread-fold" protein family; of which Escherichia coli methionine aminopeptidase is the prototype. The C-2399A variant in XPNPEP2 is associated with reduced APP activity and a higher incidence of AE-ACEi. XPNPEP2 mRNA was detected in fibroblasts that carry the translocation; suggesting that this gene at least partially escapes X inactivation. XPNPEP2 is a candidate gene for premature ovarian failure (POF).