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Recombinant Mouse HP protein (His tag)
SKU: PDEM100049-100
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Recombinant Mouse HP protein (His tag)
| SKU # | PDEM100049 |
| Expression Host | E. coli |
Description
| Synonyms | HP;BP;HPA1S;Haptoglobin;Hp |
| Species | Mouse |
| Expression_host | E.coli |
| Sequence | Ala 118-Cys 281 |
| Accession | Q61646 |
| Mol_Mass | 17.9 kDa |
| AP_Mol_Mass | 22 kDa |
| Tag | N-His |
| Bio_Activity | Not validated for activity |
Properties
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin level | Please contact us for more information. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis |
Background
Haptoglobin is a 45?48 kDa acute phase glycoprotein that is a naturally inactive member of the peptidase S1 family of serine proteases. It is secreted by multiple cell types and protects tissues from hemoglobin-mediated oxidative damage. Mature mouse (pro)haptoglobin is 329 amino acids (aa) in length. Proteolytic cleavage between Arg102-Ile103 creates disulfide-linked alpha - and beta -chains, which subsequently homodimerize through alpha -chain linkages to create a tetramer. Circulating haptoglobin is 70% tetramer and 30% unprocessed (pro)haptoglobin.