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Recombinant Mouse Interleukin-13/IL-13 Protein (Active)
SKU: PKSM041322-100
Recombinant Mouse Interleukin-13/IL-13 Protein (Active)
| SKU # | PKSM041322 |
| Expression Host | E.coli |
Description
| Synonyms | BHR, Interleukin, T-Cell Activation Protein P, ALRH, BHR1, T-Cell Activation Protein P600, Interleukin-13, IL13, NC30, P600, Il-13 |
| Species | Mouse |
| Expression Host | E.coli |
| Sequence | Pro22-Phe131 |
| Accession | P20109 |
| Calculated Molecular Weight | 13.1 kDa |
| Observed Molecular Weight | 11 kDa |
| Tag | C-His |
| Bio-activity | Measure by its ability to induce TF-1 cells proliferation. The ED50 for this effect is <4 ng/mL.The specific activity of recombinant mouse IL-13 is > 2.5 x 105 IU/mg. |
Properties
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 0.1 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from sterile PBS, pH 7.4. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
Mouse interleukin 13 (mIL-13) is a pleiotropic cytokine produced by activated Th2 cells. IL-13 induces B cell proliferation and immunoglobin production. It contains a four helical bundle with two internal disulfide bonds. Mouse IL13 shares 58% sequence identity with human protein and exhibits cross-species activity. IL13 signals via receptor IL13R (type2, IL4R) and activates STAT-6. IL13 initially binds IL-13Rα1 with low affinity and triggers association of IL4Rα, generating a high affinity heterodimeric receptor IL13R and eliciting downstream signals. IL13 also binds IL-13Rα2 with high affinity, which plays a role in a negative feedback system of IL13 signaling. IL13 is an important mediator of allergic inflammation and disease.