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Recombinant Mouse TNFα Protein(His tag)
SKU: PDMM100005-100
Recombinant Mouse TNFα Protein(His tag)
| SKU # | PDMM100005 |
| Expression Host | HEK293 Cells |
Description
| Synonyms | Cachectin, ICD1, ICD2, N-terminal fragment, NTF, TNF-a, TNF-alpha, Tumor necrosis factor ligand superfamily member 2 |
| Species | Mouse |
| Expression Host | HEK293 Cells |
| Sequence | Gly57-Leu235 |
| Accession | P06804 |
| Calculated Molecular Weight | 20.3 kDa |
| Observed Molecular Weight | 40-50 kDa |
| Tag | C-His |
| Bio-activity | Not validated for activity |
Properties
| Purity | > 90% as determined by reducing SDS-PAGE. |
| Endotoxin | Please contact us for more information. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from sterile PBS, pH 7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis. |
Background
Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.Induces insulin resistance in adipocytes via inhibition of insulin-induced IRS1 tyrosine phosphorylation and insulin-induced glucose uptake. Induces GKAP42 protein degradation in adipocytes which is partially responsible for TNF-induced insulin resistance.Plays a role in angiogenesis by inducing VEGF production synergistically with IL1B and IL6.