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Recombinant Rat NEFL Protein(His tag)
SKU: PDER100024-100
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Recombinant Rat NEFL Protein(His tag)
| SKU # | PDER100024 |
| Expression Host | E. coli |
Description
| Synonyms | NF-L;68 kDa neurofilament protein;Neurofilament triplet L protein |
| Species | Rat |
| Expression_host | E.coli |
| Sequence | Phe281-Gly396 |
| Accession | P19527 |
| Mol_Mass | 13 kDa |
| AP_Mol_Mass | 13 kDa |
| Tag | N-His |
| Bio_Activity | Not validated for activity |
Properties
| Purity | > 80 % as determined by reducing SDS-PAGE. |
| Endotoxin level | Please contact us for more information. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis |
Background
Neurofilaments usually contain three intermediate filament proteins: NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks.