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SARS-CoV-2 Nucleocapsid Protein IgM ELISA Kit
SKU: E-EL-E601
SARS-CoV-2 Nucleocapsid Protein IgM ELISA Kit
Product Details
Properties
| Assay Type | Indirect-ELISA |
| Format | 96T |
| Assay time | 1.5h |
| Sample type | serum and plasma |
| Sample volume | 100 μL |
| Storage | 2-8℃ |
| Interpretation | Qualitative |
| Application | This ELISA kit applies to the in vitro Qualitative determination of SARS-CoV-2 IgM in serum or plasma. |
| Reproducibility | Both intra-CV and inter-CV are < 10% |
| Synonyms | COVID 19 IgM,N protein IgM |
Test Principle
This ELISA kit uses Indirect-ELISA as the method to qualitatively detect the SARS-CoV-2 Nucleocapsid protein IgM in the sample. The micro ELISA plate provided in this kit is pre-coated with purified SARS-CoV-2 Nucleocapsid protein (E-ELC-001) antigen, after adding samples to wells, the SARS-CoV-2 Nucleocapsid protein IgM in the samples will combine with the pre-coated SARS-CoV-2 Nucleocapsid protein antigen. After washing completely, add Horseradish Peroxidase (HRP) conjugated mouse anti human IgM to develop the antigen-antibody-HRP conjugated secondary antibody complex. Free components are washed away, then the substrate solution is added to each well. Only those wells that contain SARS-CoV-2 Nucleocapsid protein IgM and HRP conjugated anti-human IgM will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. Compared with the CUT OFF value to judge whether SARS-CoV-2 Nucleocapsid protein IgM exists in the tested samples or not.
Kit Components and Storage
The unopened kit can be stable for 6 months at 2-8℃. After opening the kit, keep the
reagents according to the conditions below.
| Item | Specifications | Storage |
| Micro ELISA Plate(Dismountable) | 96T: 8 wells ×12 strips | 2-8℃, 6 months |
| 48T: 8 wells ×6 strips | ||
| Positive Control | 96T: 2 vials | |
| 48T: 1 vial | ||
| Negative Control | 96T: 2 vials | |
| 48T: 1 vial | ||
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL | -20℃ (Protect from light), 6 months |
| 48T: 1 vial, 60 μL | ||
| Sample & Control Diluent | 2 vial, 20 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | |
| Concentrated Wash Buffer(25×) | 1 vial, 30 mL | |
| Stop Solution | 1 vial, 10 mL | |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃(Protect from light) , 6 months |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
Assay Procedures
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1. Add 100µL of controls and samples to the appropriate wells. Incubate for 45 min at 37°C. |
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2. Aspirate and wash the plate for 3 times. |
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3. Add 100µL of HRP Conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times. |
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4. Add 90µL of Substrate Reagent. Incubate for about 15 min at 37°C. |
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5. Add 50µL of Stop Solution. |
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6. Read the plate at 450nm immediately. Calculation of the results. |
